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目的:通过研究Syndecan-l,E-cadherin及MMP-9蛋白在大肠癌组织的表达情况,探讨三者在大肠癌发生发展过程中的作用及临床意义。方法:应用免疫组化(SP)法检测52例大肠癌及18例对照正常大肠粘膜组织中Syndecan-1,E-cadherin及MMP-9蛋白的表达。结果:1.Syndecan-l、E-cadherin蛋白在大肠癌组织中的阳性表达率为65.38%、61.54%,明显低于止常大肠粘膜上皮(100%),两者比较差异有显著性(P<0.05);2.MMP-9蛋白往人肠癌组织表达的阳性率为86.54%,明显高于正常大肠粘膜上皮(11.11%),两者比较差异有显著性(p<0.01)。结论:Syndecan-1,E-cadherin及删P-9蛋白表达与大肠癌的生物学行为密切相关,在火肠癌发生发展过程中,Syndecan-I和E-cadherin蛋白的低表达或失表达和～IP-9蛋白的过度激活,可能参与了促进肿瘤侵袭转移的过程,联合检测Syndecan-i,E-cadherin和MMP-9,有助于大肠癌的诊断及对大肠癌转移潜能、预后的判断。
OBJECTIVE: To investigate the role of Syndecan-1, E-cadherin and MMP-9 in colorectal carcinoma and their roles in the development and progression of colorectal carcinoma. Methods: The expressions of Syndecan-1, E-cadherin and MMP-9 in 52 cases of colorectal carcinoma and 18 cases of normal colorectal mucosa were detected by immunohistochemistry (SP). The positive rates of Syndecan-1 and E-cadherin in colorectal cancer tissues were 65.38% and 61.54%, respectively, which were significantly lower than those in normal colorectal epithelium (100%) (P <0.05). 2. The positive rate of MMP-9 protein in human colorectal cancer tissues was 86.54%, which was significantly higher than that in normal colorectal mucosa (11.11%). There was significant difference between the two (p <0.01). Conclusions: The expression of Syndecan-1, E-cadherin and P-9 protein are closely related to the biological behavior of colorectal cancer. The expression of Syndecan-1 and E-cadherin protein in the process of development and progression The overexpression of IP-9 may be involved in the process of tumor invasion and metastasis. Combined detection of Syndecan-i, E-cadherin and MMP-9 may contribute to the diagnosis of colorectal cancer and its metastasis potential and prognosis .