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目的：阐明细胞外基质（ＥＣＭ）蛋白在实验性肺纤维化发病中的作用。方法：以大鼠气管内灌注博莱霉素（ＢＬＭ）和生理盐水分为两组，采用放免法和间接免疫荧光法观察纤维联结蛋白（ＦＮ）和层粘连蛋白（ＬＮ）的变化。结果：（１）ＢＬＭ组于灌注后第３～１４天，肺泡巨噬细胞（ＡＭ）分泌ＦＮ增加（Ｐ＜０．０１），肺泡隔内ＦＮ荧光增宽、紊乱；（２）灌注后第３天，支气管肺泡灌洗液（ＢＡＬＦ）中ＬＮ增加，呈渐进性升高，第２８天达峰值（Ｐ＜０．０１），３～７天肺泡隔内ＬＮ荧光增强，晚期明显增粗、紊乱。结论：活化的ＡＭ分泌ＦＮ、ＬＮ，ＦＮ主要参与肺纤维化早期病变的发生与发展，ＬＮ参与了ＢＬＭ肺纤维化的全过程。
Objective: To elucidate the role of extracellular matrix (ECM) protein in the pathogenesis of experimental pulmonary fibrosis. Methods: Rats were divided into two groups by intratracheal instillation of bleomycin (BLM) and normal saline. The changes of fibronectin (FN) and laminin (LN) were detected by radioimmunoassay and indirect immunofluorescence staining. RESULTS: (1) The FN secretion of alveolar macrophages (AM) increased (P <0.01) in BLM group from 3 to 14 days after perfusion, and the FN fluorescence in the alveolar septum was broadened and disturbed. (2) LN in bronchoalveolar lavage fluid (BALF) increased progressively and reached the peak on the 28th day (P <0.01) at 3 days. LN fluorescence in the alveolar septum was enhanced in 3-7 days, disorder. CONCLUSION: FN, LN and FN secreted by activated AM are mainly involved in the occurrence and development of early stage of pulmonary fibrosis. LN participates in the whole process of BLM pulmonary fibrosis.