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目的:探讨薯蓣皂苷干预脂多糖刺激的大鼠系膜细胞(MC)增殖及Wnt通路的作用机制。方法:体外培养大鼠MC(HBZY-1),设对照组、脂多糖(LPS)刺激组、LPS+薯蓣皂苷(安全浓度通过检测LDH释放率得到:0.1、0.5、1.0μg/mL)组。药物干预MC 48h后,采用MTT法检测各组MC的增殖情况,采用RT-PCR法及Western blot法检测Wnt4、β-catenin、转化生长因子β1(TGF-β1)基因和蛋白在各组MC中的表达情况。结果:薯蓣皂苷可以抑制LPS所致的MC增殖,下调Wnt4、β-catinin和TGF-β1 mRNA和蛋白水平的表达(P<0.01,P<0.05),并且在安全浓度范围内其抑制作用与剂量呈正相关。结论:薯蓣皂苷可能通过抑制Wnt信号通路,下调TGF-β1表达发挥抑制大鼠MC增殖的作用。
Objective: To investigate the effects of diosgenin on the proliferation of rat mesangial cells (MCs) stimulated by lipopolysaccharide and the mechanism of Wnt pathway. Methods: Rat MCZ (HBZY-1) was cultured in vitro. The rats in control group, LPS stimulation group and LPS + diosgenin (0.1, 0.5, 1.0 μg / mL) The proliferation of MCs was detected by MTT assay after MC 48h treatment. The expressions of Wnt4, β-catenin and TGF-β1 mRNA and protein in MC were detected by RT-PCR and Western blot, respectively The expression of the situation. Results: Diosgenin could inhibit the proliferation of MCs induced by LPS and down-regulate the mRNA and protein expression of Wnt4, β-catinin and TGF-β1 (P <0.01, P <0.05) Was positively correlated. Conclusion: Diosgenin may inhibit the proliferation of rat MC by inhibiting the Wnt signaling pathway and down-regulating the expression of TGF-β1.