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采用缓冲液/正己烷为反应体系,比较了几类表面活性剂(CTAB,SDS,SDBS,Tween 20)对酶活的影响.并在该反应体系中,考察了表面活性剂对β-葡萄糖苷酶催化合成红景天苷的反应中底物转化率以及初始反应速率的影响,确定了反应混合体系的适宜含水量.结果表明,在水/有机两相体系中,HLB值较高的SDS对酶的失活体现出了一定的抑制作用,其余表面活性剂均对酶失活起了不同程度的加速作用.在几类表面活性剂各自最适添加浓度下,CTAB,SDS,Tween 20三组均将底物转化率从9.2%提高到11%左右.不添加表面活性剂所测得的初始反应速率最高,Tween 20组次之,离子型表面活性剂添加组最低.对于SDS添加组,当其含水量为10%时,底物转化率可以达到22.3%.
The effects of several surfactants (CTAB, SDS, SDBS, Tween 20) on the enzyme activity were investigated by using buffer / n-hexane as the reaction system.In the reaction system, Enzyme catalyzed the reaction of salidroside synthesis substrate reaction rate and the initial reaction rate to determine the appropriate water content of the reaction mixture system.The results showed that in the water / organic two-phase system, the HLB value higher SDS Enzyme inactivation showed a certain inhibition, the rest of the surfactant inactivation of enzymes played different degrees of acceleration.In the optimum concentration of each surfactant, CTAB, SDS, Tween 20 three groups The conversion rate of substrate was increased from 9.2% to 11% .The initial reaction rate was the highest without Tween 20, followed by the lowest concentration with ionic surfactant.In SDS addition group, When the water content is 10%, the substrate conversion rate can reach 22.3%.