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目的探讨卤夫酮预防大鼠肝硬化的效果和机制。方法60只雌性SD大白鼠随机分为3组,每组20只,分别建立正常组、肝硬化组和卤夫酮组动物模型。采用RT-PCR法检测3组肝脏Ⅰ型胶原、金属蛋白酶组织抑制剂(TIMP)-1和α-肌球蛋白(α-SMA)的mRNA表达。结果RT-PCR检测显示肝硬化组Ⅰ型胶原、TIMP-1和α-SMA分别较正常组明显升高,差异有统计学意义(P<0.01),而卤夫酮组较肝硬化组显著降低,差异有统计学意义(P<0.01);3项指标在正常组和卤夫酮组间差异无统计学意义(P>0.05)。结论卤夫酮通过抑制Ⅰ型胶原、TIMP-1和α-SMA mRNA的转录,具有预防肝纤维化或肝硬化的作用。
Objective To investigate the effect and mechanism of halofugin on prevention of liver cirrhosis in rats. Methods Sixty female SD rats were randomly divided into three groups (n = 20 each). Animal models of normal group, cirrhosis group and halofuginone group were established respectively. The mRNA expression of type Ⅰ collagen, tissue inhibitor of metalloproteinase (TIMP) -1 and α-SMA in liver were detected by RT-PCR. Results RT-PCR showed that the expressions of type I collagen, TIMP-1 and α-SMA in cirrhosis group were significantly higher than those in normal group (P <0.01), while those in halofumarate group were significantly lower than those in cirrhosis group , The difference was statistically significant (P <0.01). There was no significant difference in the three indexes between the normal group and the halofuginone group (P> 0.05). Conclusion Halofuginone can prevent liver fibrosis and cirrhosis by inhibiting the transcription of type Ⅰ collagen, TIMP-1 and α-SMA mRNA.