论文部分内容阅读
目的观察慢性肾病对大鼠骨折愈合早期骨痂质量及生物力学的影响。方法将40只雄性SD大鼠随机分成实验组及对照组,每组20只。实验组给予含0.75%的腺嘌呤饲料喂养建立慢性肾病大鼠模型,对照组给予正常饮食。4周后,两组大鼠于右侧胫骨中段制作开放骨折模型,克氏针内固定,术后予以正常饲料喂养。骨折造模术后4周,过量麻醉处死动物,切取右侧胫骨进行生物力学测试及Micro CT扫描。结果骨折后4周,生物力学测试显示,实验组胫骨最大载荷[(34.85±9.39)N]较对照组[(46.42±3.26)N]降低24.9%(P<0.05);强度[(47.65±5.64)N·mm]较对照组[(103.79±4.12)N/mm]降低54.1%(P<0.05);能量吸收[(7.17±4.47)N·mm]较对照组[(14.25±2.21)N·mm]下降49.7%(P<0.05)。Micro CT分析显示实验组骨痂组织总量[(18.45±2.11)mm3]较对照[(29.60±2.45)mm3]组下降37.7%(P<0.05);骨性骨痂量[(7.12±3.40)mm3]较对照组[(15.65±1.32)mm3]下降54.50%(P<0.05);骨体积分数[(40.77±2.27)%]较对照组[(44.65±2.12)%]下降8.9%(P<0.05)。结论慢性肾病对大鼠骨折愈合早期骨痂质量及力学强度均有不良影响。
Objective To observe the effects of chronic kidney disease on callus quality and biomechanics in early period of fracture healing in rats. Methods Forty male SD rats were randomly divided into experimental group and control group, with 20 rats in each group. Experimental group was fed with 0.75% adenine diet to establish chronic kidney disease rat model, the control group given normal diet. Four weeks later, the two groups of rats were made an open fracture model in the right tibia. The Kirschner wire was fixed in the middle part of the tibia. After the operation, the rats were fed with normal feed. Four weeks after fracture modeling, animals were over-anesthetized and the right tibia was dissected for biomechanical testing and Micro-CT scanning. Results After 4 weeks of fracture, the biomechanical test showed that the maximum load of tibia in experimental group was (34.85 ± 9.39) N lower than that in control group (46.42 ± 3.26) N (24.9%, P <0.05), and the intensity was (47.65 ± 5.64 ) Compared with the control group [(103.79 ± 4.12) N / mm] (P <0.05); energy absorption [(7.17 ± 4.47) N · mm] mm] decreased 49.7% (P <0.05). Micro CT analysis showed that the total amount of callus in experimental group [(18.45 ± 2.11) mm3] decreased by 37.7% (P <0.05) compared with that in control [(29.60 ± 2.45) mm3] (40.77 ± 2.27)% compared with [(44.65 ± 2.12)%] in control group decreased by 54.50% (P <0.05), compared with control group [(15.65 ± 1.32) mm3] 0.05). Conclusion Chronic kidney disease has an adverse effect on the quality and mechanical strength of callus at the early stage of fracture healing in rats.