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目的:探索胆盐(GCDA)环境下肝癌细胞株HepG2的生长情况及其对抗肿瘤药物的作用研究。方法:用GCDA处理HepG2细胞,采用MTT法检测细胞活性与增殖性;用抗肿瘤药物与/或GCDA对HepG2细胞进行处理,采用流式细胞术检测凋亡率,用WesternBlotting技术检测Bcl-2家族凋亡调控蛋白的表达变化。结果:GCDA能使HepG2细胞增殖;GCDA使抗凋亡基因Mcl-1增强,促凋亡基因Bak、Bim与Bax减弱;GCDA使抗肿瘤药物作用下细胞凋亡率明显下降(P<0.05)。结论:GCDA能诱导肝癌细胞株HepG2细胞存活与增殖,并通过上调抗凋亡蛋白和下调促凋亡蛋白对抗肿瘤药物产生耐药。
Objective: To explore the growth of hepatocellular carcinoma cell line HepG2 under the condition of gallbladder salt (GCDA) and its effect on anti-tumor drugs. Methods: HepG2 cells were treated with GCDA, cell viability and proliferation were detected by MTT assay. HepG2 cells were treated with anti-tumor drug and / or GCDA. The apoptosis rate was detected by flow cytometry. Western Blotting was used to detect the expression of Bcl-2 family Apoptotic regulatory protein expression changes. Results: GCDA enhanced the proliferation of HepG2 cells; GCDA enhanced the expression of anti-apoptotic gene Mcl-1 and decreased the expression of pro-apoptotic genes Bak, Bim and Bax; GCDA decreased the apoptotic rate of cells under the action of antitumor agents (P <0.05). CONCLUSION: GCDA can induce the survival and proliferation of HepG2 cell line, and develop drug resistance to the anti-tumor drug by up-regulating anti-apoptotic protein and down-regulating pro-apoptotic protein.