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目的:探讨MMP-14蛋白在胃癌组织中的表达及在腹腔转移中的临床意义。方法:应用RT-PCR法检查98例原发性胃癌腹腔冲洗液中MMP-14的表达,30例良性病变患者腹腔冲洗液作为阴性对照。免疫组织化学SP法检测98例原发性胃癌和20例正常胃黏膜组织MMP-14表达。结果:腹腔冲洗液中MMP-14阳性表达率分别为40.8%(40/98),高于对照组的3.33%(1/30)(P<0.01)。胃癌组织中MMP-14的阳性表达率为76.5%(58/98),显著高于正常胃黏膜组织的5%(1/20)(P<0.01)。MMP-14在胃癌组织中的阳性表达率与患者的性别、年龄、分化程度无相关性(P>O.05),而MMP-14的阳性表达率与胃癌的肿瘤浸润深度、肿瘤大小、淋巴转移、大体分型、临床肿瘤分期有关(P<0.05,P<0.01)。结论:胃癌组织中MMP-14高表达,结合在腹腔冲洗液中MMP-14 mRNA的高表达,MMP-14参与了胃癌的发生发展和腹腔转移的过程。
Objective: To investigate the expression of MMP-14 in gastric carcinoma and its clinical significance in peritoneal metastasis. Methods: The expression of MMP-14 in 98 cases of primary gastric cancer with peritoneal irrigating fluid was detected by RT-PCR, and 30 cases of benign lesion were used as negative control. Immunohistochemical SP method was used to detect the expression of MMP-14 in 98 cases of primary gastric cancer and 20 cases of normal gastric mucosa. Results: The positive expression rate of MMP-14 in peritoneal washings was 40.8% (40/98), which was higher than that in the control group (3.30%, 1/30) (P <0.01). The positive expression rate of MMP-14 in gastric cancer tissue was 76.5% (58/98), which was significantly higher than that in normal gastric mucosa (1/20) (P <0.01). The positive expression rate of MMP-14 in gastric cancer tissues had no correlation with the gender, age and differentiation (P> 0.05), while the positive expression rate of MMP-14 was correlated with the depth of tumor invasion, tumor size, lymph node Metastasis, general classification, and clinical stage (P <0.05, P <0.01). Conclusion: The high expression of MMP-14 in gastric cancer tissues, combined with the high expression of MMP-14 mRNA in peritoneal washings, and the involvement of MMP-14 in the carcinogenesis and peritoneal metastasis of gastric cancer.