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目的研究赖氨酰氧化酶(LOX)在乳腺癌组织、正常乳腺组织及乳腺良性病变组织中的表达,并与乳腺癌常规预后指标比较,探讨其与乳腺癌侵袭/转移的关系。方法逆转录聚合酶链式反应(RT-PCR)检测56例乳腺癌组织、56例正常乳腺组织及20例乳腺良性病变组织中LOX mRNA与低氧诱导因子1(HIF-1α)mRNA的表达;应用免疫组织化学技术检测相应组织中LOX蛋白及HIF-1α的表达,并行统计学分析。结果 LOX mRNA在乳腺癌组织、正常乳腺组织及乳腺良性病变组织中的表达率分别为57.14%、32.14%、25.00%,乳腺癌组织中LOXmRNA的表达率明显高于正常乳腺组织及乳腺良性病变组织(P<0.01);LOX蛋白在乳腺癌组织、正常乳腺组织及乳腺良性病变组织中的表达率分别为48.21%、26.78%、20.00%,乳腺癌组织中LOX蛋白的表达率明显高于正常乳腺组织及乳腺良性病变组织(P<0.05);LOX mRNA及LOX蛋白在不同临床分期及淋巴结转移中的表达率差异有统计学意义(P<0.05),其中Ⅲ、Ⅳ期乳腺癌LOX mRNA及LOX蛋白的表达率明显高于Ⅰ、Ⅱ期(P均<0.01),但在不同年龄及肿瘤大小患者的癌组织中LOX mRNA及LOX蛋白的表达率差异无统计学意义(P>0.05);LOX蛋白的表达与HIF-1α呈正相关(r=0.368)。结论 LOX蛋白及LOX mRNA在乳腺癌组织中的表达率高于正常乳腺组织及乳腺良性病变组织,LOX与乳腺癌的发生可能有关;LOX高表达的乳腺癌其恶性程度更高,更易发生远处转移;HIF-1α与LOX在乳腺癌的侵袭/转移过程中具有协同作用。
Objective To investigate the expression of lysyl oxidase (LOX) in breast cancer tissues, normal breast tissues and benign breast lesions, and to compare with the conventional prognostic indicators of breast cancer to explore its relationship with the invasion / metastasis of breast cancer. Methods The expressions of LOX mRNA and hypoxia inducible factor 1 (HIF-1α) mRNA in 56 breast cancer tissues, 56 normal breast tissues and 20 benign breast lesions tissues were detected by RT-PCR. The expression of LOX protein and HIF-1α in corresponding tissues was detected by immunohistochemistry and statistically analyzed. Results The positive rates of LOX mRNA in breast cancer tissues, normal breast tissues and benign breast lesions were 57.14%, 32.14%, 25.00% respectively. The expression of LOX mRNA in breast cancer tissues was significantly higher than that in normal breast tissues and benign breast lesions (P <0.01). The expression rates of LOX protein in breast cancer tissues, normal breast tissues and benign breast lesions were 48.21%, 26.78%, 20.00% respectively. The expression of LOX protein in breast cancer tissues was significantly higher than that in normal breast tissues (P <0.05). The expressions of LOX mRNA and LOX protein in different clinical stages and lymph node metastasis were significantly different (P <0.05). The expression of LOX mRNA and LOX in stage Ⅲ and Ⅳ breast cancer tissues were significantly higher than those in benign breast tissues (P <0.01). However, there was no significant difference in the expression rates of LOX mRNA and LOX protein in cancer tissues of different age and tumor size (P> 0.05). LOX Protein expression was positively correlated with HIF-1α (r = 0.368). Conclusion LOX protein and LOX mRNA expression in breast cancer tissue is higher than normal breast tissue and benign breast lesions, LOX and breast cancer may be related to the LOX high expression of breast cancer is more malignant, more prone to distant Metastasis; HIF-1α and LOX have a synergistic effect in the invasion / metastasis of breast cancer.