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目的通过观察Th1/Th2漂移与肿瘤的相关性,以及细胞因子对Th1/Th2漂移的影响,探讨肿瘤免疫治疗的机制。方法Th1/Th2细胞的检测采用酶联免疫斑点法,细胞因子的检测采用双抗体夹心ELISA法。结果胃癌病人Th1细胞阳性率降低,Th2细胞阳性率升高;Th1/Th2阳性细胞比值降低(P<0.05)。IL12+抗IL4单抗组,Th1细胞阳性率增加,Th2细胞阳性率降低,Th1/Th2比值升高;IL4+抗IFNγ单抗组Th1细胞阳性率降低,Th2细胞阳性率升高,Th1/Th2比值降低(P<0.05)。胃癌、结直肠癌和乳腺癌病人均存在Th2细胞占优势的漂移状态,Th1型细胞因子IL2和IFNγ与正常人相比显著降低(P<0.05);Th2型细胞因子IL4、IL6和IL10与正常人相比显著升高(P<0.05);同时,TNFα显著著升高(P<0.05);IL12降低,其中结直肠癌病人与正常人比差异无显著性(P>0.05),胃癌和乳腺癌病人与正常人比均有显著差异(P<0.05)。不同分化程度的肿瘤病人血清细胞因子水平随着肿瘤分化程度的降低,Th1型细胞因子的降低和Th2型细胞因子的升高更加明显(P<0.05)。结论肿瘤病人Th1细胞阳性率降低,Th2细胞阳性率升高,Th1/Th2阳性细胞比值降低,同时,Th1型细胞因子降低,Th2型细胞因子升高,表现为Th2优势的漂移状态。
Objective To explore the mechanism of tumor immunotherapy by observing the correlation between Th1 / Th2 shift and tumor, and the influence of cytokine on Th1 / Th2 shift. Methods The Th1 / Th2 cells were detected by enzyme-linked immunosorbent assay (ELISA) and the cytokines were detected by double antibody sandwich ELISA. Results The positive rate of Th1 cells and the ratio of Th1 / Th2 positive cells decreased (P <0.05) in patients with gastric cancer. The positive rate of Th1 cells and the ratio of Th1 / Th2 were increased in IL12 + anti-IL4 mAb group, the positive rate of Th1 cells and Th1 / Th2 ratio were decreased in IL4 + anti-IFNγ monoclonal antibody group (P <0.05). Th2 cells predominantly existed in gastric cancer, colorectal cancer and breast cancer patients. Th1 cytokines IL2 and IFNγ were significantly lower than those in normal controls (P <0.05); Th2 cytokines IL4, IL6 and IL10 were significantly associated with normal (P <0.05); IL12 decreased, but there was no significant difference between the patients with colorectal cancer and normal subjects (P> 0.05), gastric cancer and breast Cancer patients and normal people were significantly different (P <0.05). The levels of serum cytokines in tumor patients with different degrees of differentiation decreased with the degree of tumor differentiation, the decrease of Th1 type cytokines and the increase of Th2 type cytokines were more obvious (P <0.05). Conclusions The positive rate of Th1 cells and Th2 / Th2 positive cells in tumor patients are decreased, while the Th1 type cytokines and Th2 type cytokines are increased.