从CHO细胞培养液中提纯HBsAg是制备基因工程乙肝疫苗的关键技术,提纯的主要任务是除去细胞DNA和培养液中的杂蛋白,目前采用的是以柱层析方法为主的纯化工艺,流程为:　　收获的HBsAg纯品,其纯度和残余DNA、动物免疫效力等各项检定指标均符合规定标准,产品终收率在40%左右,此项工 Purification of HBsAg from CHO cell culture medium is the key technology for preparation of genetically engineered hepatitis B vaccine. The main task of purification is to remove the extracellular protein from cell DNA and culture broth. Currently, the purification process based on column chromatography is adopted. As follows: Harvested HBsAg pure product, its purity and residual DNA, animal immune efficacy and other test indicators are in line with the prescribed standards, the final product yield of about 40% of the workers