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从杜长大杂交猪的下丘脑中提取基因组 RNA,根据报道的猪 GHRH c DNA序列设计引物 1和引物 2 ,用反转录 -聚合酶链式反应 (RT- PCR)进行 c DNA扩增 ,获得一条 132 bp的片段 ,克隆于 p GEM-T Easy载体后进行序列分析表明 ,该片段为 GHRH基因的 c DNA,它由 132个核苷酸组成 ,编码 4 4个氨基酸组成的多肽 ,即 GHRH.本研究扩增得到的基因片段与报道的猪 GHRH基因编码区序列完全一致
Genomic RNA was extracted from the hypothalamus of Durace hybridized pigs. Primer 1 and 2 were designed according to the reported GHRH cDNA sequence of porcine pigs. The c DNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) A 132 bp fragment was obtained and cloned into p GEM-T Easy vector. Sequence analysis showed that the fragment was a c DNA of GHRH gene. It consisted of 132 nucleotides and encoded a polypeptide of 44 amino acids, GHRH The gene fragment amplified in this study exactly matches the reported coding region of porcine GHRH gene