目的研究华蟾素对膀胱癌T24细胞的体外抑制作用,探讨其对膀胱癌的临床应用价值。方法用MTT法测定不同浓度华蟾素和配伍丝裂霉素C(MMC)作用下的T24细胞24h、48h的抑制作用;用流式细胞术(flowcytometer,FCM)测定不同浓度华蟾素作用T24细胞48h后细胞周期表现。结果(1)华蟾素组:25、2.5、0.25、0.025、0.001mg/ml华蟾素作用T24细胞24h后,生长抑制率分别为52.7%、47.2%、38.9%、41.7%、33.3%;作用48h后,分别为58.3%、50.0%、47.4%、47.4%、44.8%。MMC组:0.001、0.0005mg/mlMMC作用24h后,抑制率分别为71.1%、60.6%;48h后,分别为75.0%、72.2%。配伍组:0.001mg/ml MMC与2.5mg/ml华蟾素、0.0005mg/ml MMC与2.5mg/ml华蟾素,作用24h后抑制率分别为80.6%、68.4%,两组间差异有统计学意义(P<0.05);48h后抑制率分别为84.2%、72.2%。与MMC组比较,相同MMC浓度下配伍组24h、48h抑制率差异均有统计学意义(P<0.05)。(2)0.025、0.25、2.5mg/ml华蟾素作用T24细胞48h后发现T24细胞G0/G1期分别占76.3%、78.7%、79.6%。结论华蟾素对体外T24细胞有明显抑制作用,且呈时间及浓度依赖性;具有G0/G1期阻滞作用;与MMC有协同作用;可作为中晚期膀胱癌药物治疗的一种选择。 Objective To study the inhibitory effect of cinobufacini on bladder cancer T24 cells in vitro and its clinical value in bladder cancer. Methods MTT assay was used to determine the inhibitory effect of cinobufacini on MMC-induced T24 cells 24h and 48h. Flow cytometry (FCM) was used to determine the effect of cinobufacini on T24 cells. Cell cycle after 48h cell performance. Results (1) Cinobufacini group: The growth inhibitory rates of T24 cells treated with cinobufacil for 24, 25, 25, 0.25, 0.025 and 0.001 mg / ml for 24 h were 52.7%, 47.2%, 38.9%, 41.7% and 33.3%, respectively. After 48h, they were 58.3%, 50.0%, 47.4%, 47.4% and 44.8% respectively. MMC group: 0.001,0.0005mg / ml MMC for 24 hours, the inhibition rates were 71.1%, 60.6%; after 48h, were 75.0%, 72.2%. The compatibility group: 0.001mg / ml MMC and 2.5mg / ml cinobufacini, 0.0005mg / ml MMC and 2.5mg / ml cinobufagin, 24h inhibition rate were 80.6%, 68.4%, the difference between the two groups statistics Significance (P <0.05). After 48 hours, the inhibitory rates were 84.2% and 72.2%, respectively. Compared with the MMC group, the inhibitory rates at the same MMC concentration for 24h and 48h were significantly different (P <0.05). (2) T24 cells treated with 0.025, 0.25 and 2.5 mg / ml of cinobufacine for 24 hours showed 76.3%, 78.7% and 79.6% G0 / G1 phase respectively. CONCLUSION: Cinobufacini has a significant inhibitory effect on T24 cells in vitro and in a time and concentration dependent manner. It has the function of G0 / G1 arrest and synergistic effect with MMC. It can be used as an alternative medicine for the treatment of advanced bladder cancer.