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目的观察原头蚴在含不同浓度胰蛋白酶培养液中的存活、生长及其发育情况,为研究细粒棘球绦虫原头蚴发育提供基础资料。方法采集自然感染细粒棘球蚴的绵羊肝脏,在无菌条件下收集包囊内的原头蚴。将收集到的原头蚴分为6组,其中1~5组为实验组,第6组为空白对照组。在1~5组培养液内分别加入浓度为0.00%、0.25%、0.50%、1.00%和2.00%的胰蛋白酶,并加入0.70%的牛磺酸鹅脱氧胆盐(TCDC),置37℃培养箱中培养。每隔2~3d观察一次,并取原头蚴经伊红染色后,置于显微镜下计数并计算存活率。另取适量标本于解剖镜下观察虫体活力。结果在无胰蛋白酶条件下原头蚴平均存活率为98.52%,虫体活力最好;在1.00%胰蛋白酶条件下原头蚴平均存活率为92.47%,虫体活力最差,部分虫体中颗粒外溢。结论原头蚴在不同浓度胰蛋白酶作用下,其活力有不同程度的改变,以≤0.50%的胰蛋白酶对原头蚴发育的刺激作用较强,原头蚴对0.50%~1.00%胰蛋白酶的耐受性低。
Objective To observe the survival, growth and development of prothalliola in trypsin culture medium containing different concentrations, and to provide basic information for the study of the development of protoscolex. Methods Sheep liver naturally infected with Echinococcus granulosus was collected, and the protocereocystis was collected under sterile conditions. The collected primary demersor cercariae were divided into 6 groups, of which 1 to 5 groups for the experimental group, the sixth group as a blank control group. Trypsin at concentrations of 0.00%, 0.25%, 0.50%, 1.00%, and 2.00% were added to 1 to 5 groups of culture medium, and 0.70% tocopherol tauride (TCDC) Culture in the box. Every 2 ~ 3d observed once, and take the original cercaria by eosin staining, placed under the microscope count and calculate the survival rate. Another appropriate amount of specimens in the dissecting microscope observation of parasites vitality. Results The average survival rate of protocereriae in the absence of trypsin was 98.52%, and the viability of the larvae was the best. The average survival rate of protocereriae in the condition of 1.00% trypsin was 92.47% Particle spill. Conclusion Prototheca could change the vitality to varying degrees under different concentrations of trypsin. The stimulation of protozoa with ≤0.50% trypsin was strong, while the protoscolex had no effect on the growth of E.coli from 0.50% to 1.00% trypsin Low tolerance.