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A highly sensitive ultra-high-performance liquid chromatographic-tandem mass spectrometry method (UHPLC/MS/MS) was developed for the quantification of the synthetic peptide drug of exenatide in monkey plasma. Sample preparation was carried out by solid-phase extraction (SPE),and bivalirudin was used as the internal standard (I. S.).An excellent chromatographic separation was obtained on a reversed-phase C18 column with a gradient elution. Detection utilized a Qtrap 5500 μL system operated in the positive ion mode with multiple reaction monitoring (MRM).The proposed method was validated by assessing the specificity, linearity, intra-and inter-day precision and accuracy, recovery, and stability. The method resulted in a linear calibration range of 0. 10-30 ng/mL, extracting with only 50 monkey plasma aliquots. The intra- and inter-day precision (as relative standard deviation)were less than 7.5% and 9.6%, respectively. The method was validated and could be successfully utilized for the pharmacokinetic study of exenatide in monkeys following a single subcutaneous injection of Byetta.