【摘 要】
：
In our previous study,we found microRNA 5100(miR-5100)is overexpressed in lung cancer tissues.However the function of miR-5100 has not been reported yet.In this study,we report exogenous expression of
【机 构】
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Clinical Research Center,Affiliated Hospital of Guangdong Medical University
【出 处】
：
中国生物化学与分子生物学会2016年全国学术会议
论文部分内容阅读
In our previous study,we found microRNA 5100(miR-5100)is overexpressed in lung cancer tissues.However the function of miR-5100 has not been reported yet.In this study,we report exogenous expression of miR-5100 enhanced cell proliferation and colony formation abilities of A549 cells and promoted tumor growth in nude mice.Conversely,suppression of miR-5100 exhibited inhibitory effect on cell proliferation and colony formation in vitro and tomor growth in vivo.We also found miR-5100 promoted G1/S transition and downregulates cyclin D1 and CDK2 expression.In addition,we identified Rab6 is a potential target of miR-5100.We found overexpression of miR-5100 decreased accumulation of Rab6 in A549 cells.luciferase assay indicates miR-5100 directly bound to 3UTR of Rab6.Moreover,enforeced expression of Rab6 compromises effects of miR-5100 on cell proliferation and colony formation.In conclusion,our data demonstrate miR-5100 promotes tumor growth through promoting G1/S transition and targeting Rab6.
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