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Background: Long non-coding RNAs(lncRNAs)is a large class of ncRNA,the length is more than 200 nt,lack of open reading frame and no function of protein-coding RNA molecules.In more recent years,LncRNAs have been investigated as a new class of regulators of cellular processes,such as cell growth,apoptosis,and carcinogenesis.Growth arrest-specific transcript 5(GAS5)have been found play different regulation role in different cancers.For example,LncRNA GAS5 downregulated in renal cell cancer,prostate cancer,and glioblastoma multiforme,play s the role of anti-oncogene.on the contrary,LncRNA GAS5 overexpressed in osteosarcoma,plays the role of oncogene.Althouth in recent years there are many researches on LncRNA GAS5,the research on the roe of LncRNA GAS5 in breast cancer is rarely,so the aim of this study is to investigate the effect of LncRNA GAS5 on prolification,migration and invation and apoptpsis in breast cancer cells.Methods: To achieve our goal,we used qRT-PCR assay to detect the expression of LncRNA GAS5 in breast cancer SKBR-3,MDA-MB-231 and MCF-7 cells;SRB assay was used to measure the prolification ability of breast cancer cells;Wound healing and Transwell assay was used to dentect the ability of invation and migration in breast cancer cells;PI staining and AnnexinV/PI stainning was performed to analyse the cell cycle and apoptosis.Results: Among the three breast cancer cell Lines,the expression level of LncRNA GAS5 in SKBR-3 cell is lowest and the expression level of LncRNA GAS5 in MCF-7 cell is highest;After transfected MCF-7 or SKBR-3 cells with si-GAS5 or pcDNA-GAS5-vertor,the expression level of LncRNA GAS5 in MCF-7 cells is down-regulated and in SKBR-3 cells is up-regulated;depletion of LncRNA GAS5,the prolification,invation and migration ability of MCF-7 cells is increased,induced the cell cycle transformated from G0/G1 spage to S spage and inhibited apoptosis;Overexpression of LncRNA GAS5 made the prolification,invation and migration ability of SKBR-3 cells decreased,arrest cell cycle and induce apoptosis.Conclusions: lncRNA GAS5 is a novel molecule involved in breast cancer progression,which provide a potential therapeutic target.