【摘 要】
：
To understand well the mechanism involved in ethylene-induced chilling tolerance in harvested banana fruit,a gel-based proteomic study followed MALDI-TOF-TOF MS was carried out.Banana fruit were treat
【机 构】
：
Key Laboratory of Plant Resources Conservation and Sustainable Utilization,South China Botanical Gar
【出 处】
：
2015 International Conference on Fruit Quality Biology (第二届果
论文部分内容阅读
To understand well the mechanism involved in ethylene-induced chilling tolerance in harvested banana fruit,a gel-based proteomic study followed MALDI-TOF-TOF MS was carried out.Banana fruit were treated with 500 ppm ethylene for 12 h and then stored at 6 ℃.During cold storage,the proteins of fruit peel were extracted and subjected to proteomic analysis.It is observed that ethylene pretreatment significantly induced chilling tolerance in harvested banana fruit,manifesting as decreased electrolyte leakage and increased variable: maximal chlorophyll fluorescence(Fv/Fm).Sixty-four proteins spots with significant differences in abundance were identified.Most of the identified proteins were induced by ethylene pretreatment under cold stress.The up-regulated proteins by ethylene treatment mainly were related to energy metabolism,stress response and defense,methionine salvage cycle and protein metabolism.These proteins were involved in ATP synthesis,ROS scavenging,protective compounds synthesis,protein refolding and degradation,and polyamine biosynthesis.It is suggested that these up-regulated proteins might play a role in the ethylene-induced chilling tolerance in harvested banana fruit.
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