【摘 要】
：
The biocontrol efficacy to peach brown rot of fermentation liquid of Bacillus subtilis CF-3 with four preparing methods,fermentation liquid of 24 h,fermentation liquid of 7 days,bacteria-free filtrate
【机 构】
：
School of Life Sciences,Shanghai University,Shanghai 200444,China
【出 处】
：
2015 International Conference on Fruit Quality Biology (第二届果
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The biocontrol efficacy to peach brown rot of fermentation liquid of Bacillus subtilis CF-3 with four preparing methods,fermentation liquid of 24 h,fermentation liquid of 7 days,bacteria-free filtrate of 7 days and bacterial suspension of 7 days were studied from two aspects of direct contacting and volatiles antimicrobial effects in vivo and in vitro.The results of mycelial growth inhibition demonstrated that the growth inhibiting rate of direct contacting was higher than that of volatiles significantly,there was no significant difference between the inhibition ratio of vaviable suspensions in the direct contacting bacteriostatic experiment in vitro,meanwhile,the inhibition ratio of the bacteria-free filtrate of CF-3 was significantly higher than the majority of other treatment liquids by the dual culture method of volatiles produced by multifarious liquids of CF-3.The experiment set two treatment methods,punching and soaking in vivo.The volatile bacteriostatic experiment confirmed that the disease spot diameter and the decay index of CF-3 treatment fluid(24h and 7d CF-3 fermentation liquid)were significantly lower than the control group,the biocontrol effects of 24h CF-3 fermentation liquid was better.The results of the bacteriostatic experiment with direct contacting confirmed that the disease spot diameter in punching treatment of CF-3 treatment fluid(24h and 7d CF-3 fermentation liquid,bacteria-free filtrate and bacterial suspension)was significantly less than control group and the 24h CF-3 fermentation liquid had the best antimicrobial effect,the decay index in soaking process was significantly lower than the control group and the bacteria-free filtrate had the best effect.In summary,Bacillus subtilis CF-3 and its metabolites can inhibit the outbreak of peach brown rot and delay the deterioration of fruit quality.
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