【摘 要】
：
Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef,with its endogenous synthesis regulated by Stearoyl CoA desaturase (SCD1) and diacylglycerol-acyltransferasel (
【机 构】
：
Key Lab of Animal Genetics, Breeding & Molecular Design of Jangsu Province, Yangzhou University, Yan
【出 处】
：
2011中国肉牛选育改良与产业发展国际研讨会
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Intramuscular fat (IMF) is a key parameter for evaluation of nutritional quality of beef,with its endogenous synthesis regulated by Stearoyl CoA desaturase (SCD1) and diacylglycerol-acyltransferasel (DGAT1) genes in cattle.The object of this research was to evaluate the effect of SCD1 and DGAT1 polymorphisms on IMF trait in beef cattle and to estimate the frequency distribution of SNPs in the two genes in Chinese cattle populations.The SCD1 and DGAT1polymorphisms were detected by PCR-SSCP method in Chinese Simmental cattle and their associations with IMF traits were analyzed using the General Linear Model (GLM).The frequency distribution of SNPs in SCD1 and DGAT1 genes were detected by PCR-SSCP method and analyzed in seven other cattle populations.The results showed significant associations of SNPs SCD1-878,SCD1-762, and DGAT1 10433 and 10434 with IMF (%) and SFV (kg) in Chinese Simmental cattle.A haplotype combining SCD1-878C, SCD1-762T, and DGAT1 10433 and 10434-GC had the highest IMF, marbling score and shearing force.The polymorphic investigation indicated that the frequency of SCD1-878C or SCD1-762T was significantly higher in Chinese southern cattle (Leiqiong, Yunnan High pump, BMY or Minnan Cattle) thanin Chinese northern cattle (Chinese Simmental, Luxi Cattle,Bohai Black or Chinese Holstein), while the frequency of DGAT1 10433 and 10434-GC in Chinese indigenous breed (Leiqiong, Yunnan High pump, BMY, Luxi Cattle, Bohai Black, or Minnan Cattle)was significantly lower than breeds with imported blood (Chiese Simmental or Chinese Holstein).These findings demonstrated that both the SCD1 and DGAT1 SNPs were prospect genetic markers for IMF traits, and the SCD1 SNPs could be used as a genetic marker for southern or northern blood in Chinese cattle.
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