【摘 要】
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The AMPD1 gene plays an important role in the purine nucleotide cycle and energy metabolism in skeletal muscle.In the present study, polymorphism of the AMPD1 gene was detected by PCR-SSCP and DNA seq
【机 构】
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College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecu
【出 处】
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2011中国肉牛选育改良与产业发展国际研讨会
论文部分内容阅读
The AMPD1 gene plays an important role in the purine nucleotide cycle and energy metabolism in skeletal muscle.In the present study, polymorphism of the AMPD1 gene was detected by PCR-SSCP and DNA sequencing methods in 215 individuals from Qinchuan beef cattle breed.The result of DNA sequence showed two mutations by comparisons with NC_007301.The g.19416T>C and g.19421A>G mutation were detected in intron 11 ofbovine AMPD1 gene.The sequencing results of difference genotypes showed that four kinds of haplotypes, named: A (T-A), B (T-G), C (C-A) and D (C-G); and the five diplotypes were conflated and described as: AA (T-A/T-A), BC (T-G/C-A or C-G/r-A), AC (T-A/C-A), CC (C-A/C-A) and CD (C-A/C-G).Individuals with diplotype BC was significantly associated with carcass weight (P<0.05, n=56).Animals with the new heterozygote genotype had greater carcass weight than those with the other diplotypes.They may be used as a possible candidate for marker-assisted selection in Qinchuan beef cattle breeding program.
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