【摘 要】
：
The ovate family protein named MaOFP1 was identified in banana(Musa acuminata L.AAA)fruit by a yeast two-hybrid(Y2H)method using the banana MADS-box gene MuMADS1 as bait and a 2 day postharvest(DPH)ba
【机 构】
：
Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture;Institute of Tropical Bioscien
【出 处】
：
2015 International Conference on Fruit Quality Biology (第二届果
论文部分内容阅读
The ovate family protein named MaOFP1 was identified in banana(Musa acuminata L.AAA)fruit by a yeast two-hybrid(Y2H)method using the banana MADS-box gene MuMADS1 as bait and a 2 day postharvest(DPH)banana fruit cDNA library as prey.The interaction between MuMADS1 and MaOFP1 was further confirmed by Y2H and Bimolecular Fluorescence Complementation(BiFC)methods,which showed that the MuMADS1 K domain interacted with MaOFP1.Real-time quantitative PCR evaluation of MuMADS1 and MaOFP1 expression patterns in banana showed that they are highly expressed in 0 DPH fruit,but present in low levels in the stem,which suggests that simultaneous but different expression patterns exist for both MuMADS1 and MaOFP1 in different tissues and developing fruits.Meanwhile,MuMADS1 and MaOFP1 expression was highly stimulated and greatly suppressed,respectively,by exogenous ethylene.In contrast,MaOFP1 expression was highly stimulated while MuMADS1 was greatly suppressed by the ethylene competitor 1-methylcyclopropene(1-MCP).These results indicate that MuMADS1 and MaOFP1 are antagonistically regulated by ethylene and might play important roles in postharvest banana fruit ripening.
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