PPK1 encoded by ppk1 gene,is mainly response for the synthesis of polyphosphate (PolyP),which is an important kind of in......

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应用ＰＣＲ技术从Ｅ．ｃｏｌｉＪＭ１０５中扩增出长约１．４Ｋｂ的色氨酸酶基因，将其插高表达载体ＰＥＴ３ａ的ＮｄｅＩ／ＢａｍＨＩ位点，转化Ｅ．ｃｏｌｉＢＬ２１（ＤＥ３），构建高产色氨酸酶基因工程菌。......

以邻硝基苯胺为起始原料，经邻硝基苯的重氮化，Ｌ－衬胱氨酸与邻硝基苯重氮盐的取代反应等步骤制备ＳＯＰＣ，通过活性炭吸附，氨水洗脱和硅胶柱层析纯......