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报告用简单的燃烛放干燥缸的培养方法及洗除抗冻剂的方法,使超低温(-196℃)保存的人恶性疟原虫获得连续培养已300多天,原虫繁殖较好,电镜观察表明原虫结构属正常。对于疟原虫连续培养成功的可能因素和有关问题进行了讨论。人恶性疟原虫连续培养的成功,为疟疾疫苗研制工作提供了良好条件。
Report with a simple method of burning candle drying drier and anti-freezing method of removing cryogenic (-196 ℃) preserved P. falciparum continuous culture has been obtained for more than 300 days, protozoan reproduction is better, electron microscopy showed that Protozoa structure is normal. The possible causes of success of continuous culture of Plasmodium and related problems are discussed. The success of continuous culture of human Plasmodium falciparum provides favorable conditions for the development of malaria vaccine.