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目的探讨人类白细胞抗原HLA-DRB1基因多态性与新疆宫颈癌高发区和田维吾尔族妇女宫颈癌的关系。方法采用聚合酶联反应序列特异性寡核苷酸探针(Polymerase chain reaction sequence specific oligonucleotide,PCR-SSO)法对92例新疆和田维吾尔族宫颈癌患者及92例正常妇女宫颈组织中检测HLA-DRB1的等位基因。结果HLA-DRB1*15在宫颈癌组中出现的频率明显高于对照组,并有统计学差异(P<0.05);HLA-DRB1*03和HLA-DRB1*08在宫颈癌组中出现的频率明显低于对照组,并有统计学差异(P<0.05);HLA-DRB1的其他等位基因在宫颈癌组及对照组中出现的频率无显著性差异(P>0.05)。结论HLA-DRB1*15可能与新疆和田维吾尔族妇女对宫颈癌的遗传易感性有关,是维吾尔族妇女对宫颈癌的易感基因;而HLA-DRB1*03和HLA-DRB1*08可能为新疆和田维吾尔族妇女宫颈癌的保护基因,维吾尔族妇女宫颈癌易感基因及保护基因的检测可能在高危人群的检测及群体遗传干预工作中有一定的指导意义。
Objective To investigate the relationship between human leukocyte antigen HLA-DRB1 gene polymorphism and cervical cancer in Xinjiang and Xinjiang Uygur women. Methods Polymerase chain reaction sequence specific oligonucleotide (PCR-SSO) method was used to detect the expression of HLA-DRB1 in 92 cases of Uygur cervical cancer patients and 92 normal women in Xinjiang Uygur Autonomous Region. Of alleles. Results The frequencies of HLA-DRB1 * 15 in cervical cancer group were significantly higher than those in control group (P <0.05). The frequencies of HLA-DRB1 * 03 and HLA-DRB1 * 08 in cervical cancer group (P <0.05). There was no significant difference in the frequencies of other HLA-DRB1 alleles between cervical cancer group and control group (P> 0.05). Conclusion HLA-DRB1 * 15 may be related to the genetic susceptibility of cervical cancer to Uygur women in Hetian, Xinjiang and is a susceptible gene to cervical cancer in Uyghur women. HLA-DRB1 * 03 and HLA-DRB1 * 08 may be susceptible to cervical cancer in Xinjiang Uygur women cervical cancer protection genes, Uygur women with cervical cancer susceptibility genes and protective genes may be detected in high-risk groups and population genetic intervention in a certain degree of guiding significance.