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为探讨模拟失重条件下Ｔ淋巴细胞功能改变的机制，观察了小鼠脾脏Ｔ淋巴细胞增殖能力（ＭＴＴ比色法）、ＩＬ－２产生（生物学测定法）、ＩＬ－２基因（点杂交）和Ｂｃｌ－２癌基因（逆转录ＰＣＲ）转录的变化。结果显示：（１）模拟失重７、１４ｄ小鼠脾脏Ｔ淋巴细胞增殖能力和ＩＬ－２的产生降低，１４ｄ时有显著性（Ｐ＜０．０１）。表明模拟失重降低Ｔ淋巴细胞功能。（２）模拟失重７、１４ｄ小鼠脾脏Ｔ淋巴细胞ＩＬ－２和Ｂｃｌ－２基因转录水平降低，１４ｄ时有显著性（分别为Ｐ＜０．０５和Ｐ＜０．０１）。表明模拟失重通过抑制ＩＬ－２基因转录抑制ＩＬ－２的产生，ＩＬ－２和Ｂｃｌ－２基因对模拟失重引起的Ｔ淋巴细胞功能的降低起一定的作用。
To investigate the mechanism of T lymphocyte function changes under simulated weightlessness, the effects of MTT colorimetric assay, IL-2 production (biological assay), IL-2 gene (dot blot) And Bcl-2 oncogene (reverse transcription PCR). The results showed that: (1) T lymphocyte proliferation and IL-2 production in the spleens of mice on the 7th and 14th day after simulated weightlessness decreased significantly (P <0.01) on the 14th day. Show that simulated weight loss reduces T lymphocyte function. (2) The transcriptional levels of IL-2 and Bcl-2 in splenic T lymphocytes of mice on the 7th and 14th day after simulated weightlessness decreased significantly (P <0.05 and P <0.01, respectively). These results suggest that simulated weightlessness inhibits the production of IL-2 by inhibiting the transcription of IL-2 gene. IL-2 and Bcl-2 genes play a role in the reduction of T lymphocyte function induced by simulated weightlessness.