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氨气是造成家禽呼吸道疾病的主要污染气体,它能引起气管损伤,而该损伤机理还未见详细报道.因此,本研究采用同位素标记相对和绝对定量(i TRAQ)技术,研究高浓度氨气刺激下艾拔益加肉鸡(Gallus domesticus)(简称AA肉鸡)气管的蛋白质表达变化,以揭示氨气对气管损伤的潜在机理.本研究共鉴定3706个蛋白(FDR<1%),其中119个蛋白差异表达.通过GO功能分析发现,参与免疫应答和肌肉收缩的蛋白显著富集.在免疫应答类别中,上调蛋白(FGA等)与促炎效应相关,而下调蛋白参与抗原处理和提呈(MYO1G等)、免疫球蛋白和抗菌肽的生成(fowlicidin-2等)、以及免疫缺陷(PTPRC等)过程.同时在肌肉收缩过程发挥重要作用的蛋白(TPM1等)均表达上调.作为呼吸道疾病的共同特征之一,本研究也发现了黏蛋白的高表达.最后,通过实时RT-PCR对6个差异蛋白的表达水平进行验证.研究结果不仅提示高氨刺激可能造成气管阻塞、降低宿主防御功能,也为将来抵御氨气毒性的措施提供了线索.
Ammonia is the main pollutant gas that causes respiratory diseases in poultry and it can cause tracheal injury, but the mechanism of this injury has not been reported yet.In this study, isotope labeled relative and absolute quantification (i TRAQ) The protein expression of trachea in Gallus domesticus (AA broiler) was stimulated to reveal the potential mechanism of ammonia on tracheal injury.In this study, a total of 3706 proteins (FDR <1%) were identified, of which 119 Protein differential expression.Functional analysis of GO found that proteins involved in immune response and muscle contraction were significantly enriched.In the immune response category, upregulated proteins (FGA, etc.) are associated with proinflammatory effects, whereas downregulated proteins are involved in antigen processing and presentation MYO1G, etc.), immunoglobulin and antimicrobial peptides (fowlicidin-2, etc.), immunodeficiency (PTPRC, etc.), and proteins that play an important role in muscle contraction (TPM1, etc.) One of the common features of this study also found that high expression of mucins.Finally, the expression levels of six differential proteins were verified by real-time RT-PCR.The results not only suggest that high ammonia stimulation may Cause airway obstruction, reduced host defense functions, but also provide clues to future measures against ammonia toxicity.