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目的 :探讨食管癌组织中多药耐药基因 (mdr 1)和多药耐药相关蛋白基因 (mrp)表达的意义。方法 :运用逆转录 多聚酶链反应 (RT PCR)技术检测了 32例食管癌患者的癌组织标本及癌旁组织中mdr 1和mrp表达 ,分析其与肿瘤分化程度、浸润深度及淋巴结转移的关系。结果 :32例食管癌组织中mdr 1和mrp表达的阳性率均高于癌旁组织 (P <0 0 1,P <0 0 2 5 ) ,两基因的表达水平也显著高于癌旁组织 (P <0 0 0 1,P <0 0 0 1) ;在中低分化食管癌和肿瘤侵犯深肌层或纤维层时 ,mdr 1、mrp表达水平均高于高分化肿瘤和癌肿侵犯浅肌层以内者 (P <0 0 5 ,P <0 0 1)。结论 :食管癌组织中存在着原发性多药耐药性 ,mdr 1和mrp表达增高可能提示肿瘤的分化不良 ,并可反映肿瘤的浸润深度。
Objective: To explore the significance of multidrug resistance gene (mdr 1) and multidrug resistance-associated protein gene (mrp) expression in esophageal cancer tissue. METHODS: RT-PCR was used to detect the expression of mdr 1 and mrp in 32 specimens of esophageal cancer patients and adjacent tissues. The relationship between the expression of mdr 1 and mrp and tumor differentiation, depth of invasion and lymph node metastasis was analyzed. Results: The positive rates of mdr 1 and mrp expression in 32 cases of esophageal cancer were higher than those in adjacent tissues (P < 0.01, P <0 0 25). The expression levels of the two genes were also significantly higher than those in adjacent tissues. P <0 0 0 1, P <0 0 0 1); In poorly differentiated esophageal cancer and tumor invasion of deep muscle or fibroplasia, the expression level of mdr 1 and mrp was higher than that of well-differentiated tumor and cancer invasion of superficial muscle. Within the layer (P <0 05, P <0 01). Conclusion : There is primary multidrug resistance in esophageal cancer tissues. Increased expression of mdr 1 and mrp may indicate poor tumor differentiation and reflect the depth of tumor invasion.