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目的了解浙江省伤寒副伤寒沙门菌耐药及分子分型特点,为伤寒副伤寒防制工作提供依据。方法采用WHO推荐的改良K-B纸片法,对209株甲型副伤寒沙门菌和5株伤寒沙门菌进行14种抗生素敏感性试验;运用脉冲场凝胶电泳分型(PFGE)方法对119株甲型副伤寒沙门菌进行分子分型及流行病学特征分析。结果209株甲型副伤寒沙门菌对红霉素、萘啶酸和利福平100%耐药,对阿米卡星100%敏感,对环丙沙星的敏感率仅为58%;5株伤寒沙门菌对红霉素和利福平100%耐药,对头孢噻吩、头孢噻肟、庆大霉素、环丙沙星、氨苄西林、阿米卡星和阿莫西林/克拉维酸100%敏感;不同地区间的甲型副伤寒沙门菌对大部分药物敏感率一致,只有多西环素存在较大差异;119株甲型副伤寒沙门菌共产生20种PFGE带型,并主要集中在2种同源性较高的型别。结论2008年浙江省伤寒副伤寒以甲型副伤寒菌株为优势株,不同地区甲型副伤寒沙门菌对大部分抗生素敏感率一致;高发地区(台州)的菌株PFGE带型比较分散,可能与菌株变异有关。
Objective To understand the characteristics of Salmonella typhi and Salmonella typhimurium isolates in Zhejiang Province and to provide basis for the prevention and control of typhoid and paratyphoid fever. Methods A total of 209 strains of Salmonella paratyphi A and 5 strains of Salmonella typhi were tested for susceptibility to antibiotics using the modified KB paper method recommended by the WHO. By means of pulsed-field gel electrophoresis (PFGE), 119 strains of A Salmonella paratyphi molecular typing and epidemiological characteristics analysis. Results 209 strains of Salmonella paratyphi A were 100% resistant to erythromycin, nalidixic acid and rifampicin, 100% sensitive to amikacin, and 58% to ciprofloxacin. Salmonella typhi was 100% resistant to erythromycin and rifampicin, but cefotaxime, cefotaxime, gentamicin, ciprofloxacin, ampicillin, amikacin and amoxicillin / clavulanic acid 100 %. The sensitivity of Salmonella paratyphi A to most of the drugs in different regions was the same, only the presence of doxycycline was quite different. 119 strains of Salmonella paratyphi A produced 20 PFGE patterns and mainly concentrated In the two types of higher homology. Conclusions In 2008, typhoid and paratyphoid A strain of Zhejiang Province was the dominant strain, and Salmonella paratyphi A was sensitive to most of the antibiotics in different regions. PFGE patterns of strains in high incidence area (Taizhou) Variation related.