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实验选择了一对葡萄糖转运蛋白基因（Ｇｌｕｔ４基因）外显子４α的侧翼引物，应用单链构型多态性（ＰＣＲ－ＳＳＣＰ）方法，扩增６０例非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者外周淋巴细胞基因组的ＤＮＡ，经琼脂糖凝胶电泳检测证实为２５２ｂｐ，该产物经６％非变性聚丙烯酰胺凝胶电泳检测发现１例异常带型，显示了电泳的泳动变位。进一步深入研究ＮＩＤＤＭ的遗传异质性，有助于阐明ＮＩＤＤＭ的病因及发病机制
A pair of flanking primers of exon 4α of glucose transporter gene (Glut4 gene) was selected for experiment, and 60 cases of non-insulin dependent diabetes mellitus (NIDDM) were amplified by PCR-SSCP The DNA of lymphocyte genome was confirmed to be 252bp by agarose gel electrophoresis. One of the abnormal bands was detected by 6% non-denaturing polyacrylamide gel electrophoresis, showing the electrophoretic mobility shift. Further study of the genetic heterogeneity of NIDDM will help elucidate the etiology and pathogenesis of NIDDM