角膜移植术常感到授眼之不足,尤其是全层角膜移植,所采用的角膜保存于4℃,数日后即不适用,浪费很大.作者采用低温冷冻人类角膜保存法,初步观查效果佳。材料和方法:将纯 Dimethyl Sulphoxide(DMSO)装于1毫升的密闭安甑,50%甘油蒸馏水装于2毫升 Corneal transplantation often feel the lack of eye, especially the full-thickness corneal transplantation, the cornea used stored at 4 ℃, a few days later that is not applicable, a lot of waste.Using cryogenic frozen human corneal preservation method, the initial observation of good results . MATERIALS AND METHODS: Pure Dimethyl Sulphoxide (DMSO) was placed in 1 mL of sealed ampoule and 50% glycerol distilled water in 2 mL