本研究用兔子血小板,研究各种汉方驱瘀血剂对骨胶原、花生四烯酸和凝血酶所致凝集的阻断效果,探讨其阻断机理。下述六种方剂的生药热提1h后,可溶成分喷雾干燥。干燥粉末在300ml水中悬浮,离心分离,过滤上清液,冻干。冻干粉溶于磷酸缓冲盐水(PBS)(140.5mM NaCl、10mM磷酸盐、pH7.4),供试验。 In this study, rabbit platelets were used to study the blockade effects of various Chinese herbal repellents on collagen, arachidonic acid and thrombin-induced agglutination, and the blocking mechanism was explored. The soluble extracts were spray-dried after 1 h of hot extraction of the following six formulations. The dry powder was suspended in 300 ml of water, centrifuged, and the supernatant was filtered and lyophilized. The lyophilized powder was dissolved in phosphate buffered saline (PBS) (140.5 mM NaCl, 10 mM phosphate, pH 7.4) for testing.