目的探讨过氧化氢诱导鼠白内障形成过程中晶体上皮凋亡细胞中半胱氨酸天冬氨酸酶-2(Cas-pase-2)的表达及意义。方法大鼠晶状体器官离体培养后,用免疫组织化学方法检测晶体上皮细胞Caspase-2的表达。结果随过氧化氢损伤时间的处长,晶体上皮细胞Caspase-2的表达逐渐增强,与晶体混浊程度正相关。结论过氧化氢可以诱导鼠晶体上皮细胞Caspase-2的表达,后者可能参与晶体上皮细胞凋亡和白内障的形成。 Objective To investigate the expression and significance of Caspase-2 in crystalline epithelial apoptotic cells induced by hydrogen peroxide in cataract formation. Methods After the lens organs were cultured in vitro, the expression of Caspase-2 in lens epithelial cells was detected by immunohistochemistry. Results With the length of injury time of hydrogen peroxide, the expression of Caspase-2 in lens epithelial cells gradually increased, which was positively correlated with the degree of crystal opacity. Conclusion Hydrogen peroxide can induce the expression of Caspase-2 in rat lens epithelial cells, which may be involved in the apoptosis of lens epithelial cells and the formation of cataract.