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目的： 探讨β－胡萝卜素抑制白血病细胞增殖的机制。方法： 体外培养白血病细胞株ＨＬ－６０，培养时添加１０μｍ ｏｌ／Ｌ、５０μｍ ｏｌ／Ｌ、１００μｍ ｏｌ／Ｌβ－胡萝卜素作用２４ｈ、４８ｈ、７２ｈ 后，用台盼蓝拒染法进行活细胞计数，绘制细胞生长曲线；应用流式细胞术分析细胞周期分布，同时利用流式细胞术结合ＤＮＡ 凝胶电泳检测白血病细胞凋亡。结果： 细胞生长曲线显示１０～１００μｍ ｏｌ／Ｌ的β－胡萝卜素均对白血病细胞株ＨＬ－６０ 有显著性抑制作用，且呈剂量效应关系。流式细胞术结果显示５０μｍ ｏｌ／Ｌ的β－胡萝卜素处理ＨＬ－６０ 细胞２４ｈ 后，出现Ｓ期阻滞，并诱导５．５９％ 的细胞凋亡。ＤＮＡ 凝胶电泳结果同样显示β－胡萝卜素能够诱导白血病ＨＬ－６０ 细胞凋亡。结论： β－胡萝卜素对于髓系白血病细胞株ＨＬ－６０ 细胞的增殖具有显著性抑制作用，其机制可能是干扰的白血病细胞ＤＮＡ代谢；β－胡萝卜素诱导白血病细胞凋亡也是其抑制白血病的重要途径之一。
Objective: To investigate the mechanism of β-carotene inhibiting the proliferation of leukemia cells. Methods: In vitro leukemia cell line HL-60 was incubated with 10 μm ol/L, 50 μm ol/L, and 100 μm ol/L β-carotene for 24 h, 48 h, and 72 h, and then counted by trypan blue exclusion. The cell growth curve was plotted; cell cycle distribution was analyzed by flow cytometry, and apoptosis of leukemia cells was detected by flow cytometry combined with DNA gel electrophoresis. RESULTS: The cell growth curve showed that 10-100 μm ol/L β-carotene significantly inhibited the leukemic cell line HL-60 and showed a dose-effect relationship. Flow cytometry results showed that after treatment with 50 μm ol/L β-carotene for HL-60 cells for 24 h, S phase arrest occurred and 5.59% of cells were induced to undergo apoptosis. The results of DNA gel electrophoresis also showed that β-carotene can induce apoptosis in leukemic HL-60 cells. Conclusion: β-carotene has a significant inhibitory effect on the proliferation of myeloid leukemia cell line HL-60 cells. The mechanism may be the interference of DNA metabolism in leukemia cells; β-carotene induces leukemic cell apoptosis is also important in inhibiting leukemia. One of the ways.