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目的：研究幽门螺杆菌（HP）与胃腺上皮细胞增生的关系，方法：经PCR检测和组织病理学观察138例胃粘膜活检标本，采用SP免疫组化染色观察PCNA表达和Feulgen二染色测定细胞核DNA含量。结果：细胞核DNA含量在慢性浅表性胃炎（CSG）和慢性萎缩性胃炎（CAG）中，有HP感染与无HP感染比较，二倍体和近二倍体细胞减少，增殖倍体增加。PCNA表达在CSG和CAG中，有HP感染与无HP感染比较阳性细胞数和均值增加。结论：HP感染与胃腺上皮细胞的增生程度有密切关系。
Objective: To study the relationship between Helicobacter pylori (HP) and the proliferation of gastric glandular epithelial cells. Methods: 138 cases of gastric biopsy specimens were detected by PCR and histopathology. SPNA immunohistochemistry was used to observe the expression of PCNA and Feulgen two staining to determine the nuclear DNA. content. RESULTS: The nuclear DNA content in chronic superficial gastritis (CSG) and chronic atrophic gastritis (CAG) had HP infection and no HP infection, diploid and near diploid cells decreased, and proliferative ploidy increased. PCNA was expressed in CSG and CAG. There was an increase in the number and mean number of positive cells compared to HP infection and no HP infection. Conclusion: HP infection is closely related to the degree of proliferation of gastric gland epithelial cells.