从新鲜猪粪便中分离得到一株猪源拟杆菌,利用统计学的单因素试验与正交试验,对该菌株的发酵培养基及发酵条件进行优化。实验结果表明该菌发酵最适培养基为AN培养基,确定了促生长因子吐温-80、1%氯化血红素、1%维生素Kl的最佳浓度,并确定了最佳发酵条件,最佳培养温度为37℃、分批发酵时间周期18 h。 Bacteroides aureus was isolated from fresh pig manure. The single factor test and orthogonal experiment were used to optimize the fermentation medium and fermentation conditions. The experimental results showed that the optimal medium for fermentation was AN medium, and the best concentration of growth factor Tween-80, 1% hemin and 1% vitamin Kl was determined, and the optimum fermentation conditions were determined Good culture temperature was 37 ℃, batch fermentation time period 18 h.