本法为蔬菜中硝酸盐的测定法,根据硝酸与2—异丁基苯酚在硫酸(5+7)中的定量反应,然后被充分地提取,并在碱性中形成黄色络合物而进行测定。此法反应灵敏、颜色稳定。硝酸盐的浓度在0.13～2.50μg/ml时,于418nm测定吸光度,服从比尔定律。蔬菜中存在各种可能干扰物质都不干扰硝酸盐与2—异丁基苯酚的反应。蔬菜及其制品中硝酸盐的回 This method for the determination of nitrate in vegetables is based on the quantitative reaction of nitric acid with 2-isobutylphenol in sulfuric acid (5 + 7), followed by sufficient extraction and formation of a yellow complex in basicity Determination. This method is responsive and stable in color. The absorbance was measured at 418 nm for nitrate concentrations from 0.13 to 2.50 μg / ml, subject to Beer’s law. There is a variety of possible interfering substances in vegetables that do not interfere with the reaction of nitrate with 2-isobutylphenol. Nitrate back in vegetables and their products