目的研究不同产地槐角中槐角苷的含量。方法收集了陕西、安徽、河北、山东、河南等产地样品,采用HPLC测定了其中槐角苷的含量,色谱条件:以Agilent C18柱(250 mm×4.6 mm,5μm)为色谱柱,流动相为甲醇-乙腈-0.1%磷酸水溶液(10∶20∶70),流速1 ml/min,检测波长为260 nm。结果确定的方法能准确测定槐角中槐角苷的含量。结论通过对槐角主产地样品分析表明,以河北和陕西产槐角中槐角苷的含量较高。 Objective To study the contents of acacia in Sophora japonica from different areas. Methods The samples from Shaanxi, Anhui, Hebei, Shandong and Henan were collected and the content of the acarbose was determined by HPLC. The chromatographic conditions were as follows: Agilent C18 column (250 mm × 4.6 mm, 5 μm) Methanol-acetonitrile-0.1% phosphoric acid solution (10:20:70) at a flow rate of 1 ml / min with a detection wavelength of 260 nm. Results determined by the method can accurately determine the Huaijiao in the content of acaclycidin. Conclusion Through the analysis of the samples from the main producing areas of Huaijiao, the contents of acaclycidin in Huaijiao of Hebei and Shaanxi are higher.