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目的建立晶状体连续发育模型,为进一步研究晶状体发育过程中起决定性作用的基因和因子的正常表达提供实验基础。方法雌雄昆明小鼠合笼饲养;将孕鼠分笼饲养;分别断髓处死不同胚龄的孕鼠和出生后不同年龄的小鼠;抽提不同胚龄晶状体泡的总RNA及出生后不同年龄小鼠晶状体的总RNA。结果得到胚龄9.5d(E9.5 d)、E12.5 d、E17.5 d、新生10 d、出生20 d及成年60 d小鼠晶状体的总RNA。形成了晶状体连续发育的各个阶段。结论孕鼠可以通过雌雄鼠合笼后,检查有无阴栓得知,并可以精确计算胎龄天数。因此可得到不同发育时期的鼠胚,为建立连续的晶体发育模型提供了可能。
Objective To establish a continuous lens growth model, which provides experimental basis for further study of the normal expression of genes and factors that play a decisive role in the development of lens. Methods Male and female Kunming mice were housed in cage. Pregnant rats were housed in separate cages. Pregnant mice of different embryonic ages and mice of different ages after birth were sacrificed. Total RNA of lens vesicles with different embryonic ages and different ages after birth Total RNA from mouse lens. Results The total RNA of lens in mice with embryonic age 9.5d (E9.5d), E12.5d, E17.5d, newborn 10d, born 20d and adult 60d were obtained. The formation of the lens of the various stages of continuous development. Conclusion Pregnant mice can be masked by male and female rats, check whether there is no vaginal plug, and can accurately calculate the gestational age. Therefore, we can get different stages of development of mouse embryos, to establish a continuous model of crystal growth possible.