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目的考核酵母基因工程乙肝疫苗在易感藏族人群中的免疫原性。方法为1 609名从横断面调查中筛选出的HBV易感者接种汉逊酵母乙型肝炎疫苗,剂量10ug/mL,程序0、1、6个月。免疫7个月后,随机整群选择615名接种者采集静脉血标本,固相放射免疫(RIA)法定性检测HBsAg、抗-HB,Abbott公司的MEIA试剂定量检测抗-HBs。结果共549名接种者抗-HBs阳转,阳转率89.3%,抗-HBs阳转者几何平均浓度(GMC)为3 514.6mIU/mL;抗体阳转率和GMC均呈随年龄降低的趋势,而与接种者的性别、职业、居住地等无关;观察结束时检出8名HBsAg阳转者,其原因待查。结论藏族人群对基因工程乙肝疫苗有良好的免疫应答。
Objective To evaluate the immunogenicity of yeast genetically engineered hepatitis B vaccine in susceptible Tibetan people. Methods A total of 1 609 HBV susceptible individuals screened from the cross-sectional survey were given Hansenula Hepatitis B vaccine at the dose of 10 ug / mL for 0, 1 and 6 months. Seven months after the immunization, a total of 615 vaccinated individuals were randomly selected to collect venous blood samples. RIA-based qualitative detection of HBsAg and anti-HBs was performed by Abbott’s MEIA reagent for the quantitative detection of anti-HBs. Results A total of 549 vaccinated individuals were positive for anti-HBs with a positive rate of 89.3% and an average geometric mean (GMC) of 5146 mIU / mL for anti-HBs positive mothers. The rates of antibody positive conversion and GMC decreased with age , Regardless of the sex, occupation, residence, etc. of the inoculated persons. The reason for the detection of 8 HBsAg positive persons at the end of observation is to be investigated. Conclusion The Tibetan population has a good immune response to genetically engineered hepatitis B vaccine.