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为揭示天目山毛竹入侵原始阔叶林后土壤真菌群落特征的变化,采用T-RFLP以及荧光定量PCR技术,分析毛竹纯林、竹阔混交林及原始阔叶林土壤真菌群落结构和数量特征.结果表明:土壤真菌群落结构差异在毛竹纯林和阔叶林之间最为明显,其次为竹阔混交林和阔叶林;竹阔混交林土壤具有最高的真菌Shannon指数、均匀度指数及最低的Simpson指数.硝态氮含量和p H显著影响了真菌群落结构的变异,毛竹林土壤真菌群落结构受p H和铵态氮影响较大,而阔叶林主要受硝态氮影响.阔叶林土壤真菌数量显著高于毛竹纯林和竹阔混交林,真菌数量分别与土壤p H和硝态氮呈现显著负相关和正相关.表明真菌在阔叶林土壤中介导了异养硝化作用,毛竹入侵可能对此过程产生了显著影响.
To reveal the changes of soil fungal community characteristics after invasion of virgin bamboo forests in Tianmu Mountain, T-RFLP and fluorescence quantitative PCR techniques were used to analyze the fungal community structure and quantitative characteristics of pure bamboo forest, broad-leaved bamboo forest and virgin- The results showed that the difference of soil fungal community structure was the most obvious between pure and broad-leaved forest, followed by broad-leaved forest and broad-leaved forest. The highest fungi Shannon’s index, evenness index and lowest Simpson Index.The nitrate nitrogen content and p H significantly affected the variation of fungal community structure, while the community structure of fungi in moso bamboo forest was strongly influenced by p H and ammonium nitrogen, while the broad-leaved forest was mainly affected by nitrate nitrogen. The number of fungi was significantly higher than that of pure bamboo forest and broad-leaved-mixed forest, and the numbers of fungi were significantly and negatively correlated with soil p H and nitrate nitrogen respectively, indicating that the fungi mediated heterotrophic nitrification in the broad-leaved forest soils, This process may have a significant impact.