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AIM:To investigate the protective effect of ischernicpreconditioning(IPC)on hepatocellular carcinoma(HCC)patients with cirrhosis undergoing hepatic resection underhepatic inflow occlusion(HIO)and its possible mechanism.METHODS:Twenty-nine consecutive patients with resectable0HCC were randomized into two groups:IPC group:beforeHIO,IPC with 5 min of ischemia and 5 min of reperfusionwas given;control group:no IPC was given.Liver functions,hepatic Caspase-3 activity,and apoptotic cells were comparedbetween these two groups.RESULTS:On postoperative days(POD)1,3 and 7,theaspartate transaminase(AST)and alanine transaminase(ALT)levels in the IPC group were significantly lower thanthose in the control group(P<0.05).On POD 3 and 7,thetotal bilirubin level in the IPC group was significantly lowerthan that in the control group(P<0.05).On POD 1,thealbumin level in the IPC group was higher than that in thecontrol group(P=0.053).After 1 h of reperfusion,bothhepatic Caspase-3 activity and apoptotic sinusoidal endothelialcells in the IPC group were significantly lower than thosein the control group(P<0.05).CONCLUSION:IPC has a potential protective effect onHCC patients with cirrhosis.Its protective mechanismunderlying the suppression of sinusoidal endothelial cellapoptosis is achieved by inhibiting Caspase-3 activity.
AIM: To investigate the protective effect of ischernic preconditioning (IPC) on hepatocellular carcinoma (HCC) patients with cirrhosis undergoing hepatic resection under hepatic inflow occlusion (HIO) and its possible mechanism. METHODS: Twenty-nine consecutive patients with resectable HCT were randomized into two groups: IPC group: beforeHIO, IPC with 5 min of ischemia and 5 min of reperfusion was given; control group: no IPC was given. Liver function, hepatic Caspase-3 activity, and apoptotic cells were comparedbetween these two groups .RESULTS: On postoperative days ( POD) 1, 3 and 7, the aspartate transaminase (AST) and alanine transaminase (ALT) levels in the IPC group were significantly lower thanthose in the control group (P <0.05) .On POD 3 and 7, the total bilirubin level in the IPC The group was significantly lowererthan that in the control group (P <0.05) .On POD 1, thealbumin level in the IPC group was higher than that in the control group (P = 0.053) .After 1 h of reperfusion, bothhepatic Caspase-3 activity and apoptotic si nusoidal endothelial cells in the IPC group were significantly lower than those of the control group (P <0.05) .CONCLUSION: IPC has a potential protective effect onHCC patients with cirrhosis. Its protective mechanismunderlying the suppression of sinusoidal endothelial cellapoptosis is achieved by inhibiting Caspase-3 activity .