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研究了葡萄糖氧化酶（ＧＯＤ）、β－Ｄ－葡萄糖（β－Ｄ－Ｇ）、辣根过氧化物酶（ＨＲＰ）、色原物（ＣＳ）及ｐＨ 值等因素对放大系统的影响。结果表明，放大系统的最佳ｐＨ 值为６．４，理想色原物是联大茴香胺，３，３′，５，５′－四甲基联苯胺（ＴＭＢ）和邻苯二胺（ＯＰＤ）不宜作为放大系统的色原物。ＧＯＤ，β－Ｄ－Ｇ，ＨＲＰ和ＣＳ在不同程度上均极显著（Ｐ＜ ０．０１）地影响试验结果，其主次关系为ＧＯＤ＞ β－Ｄ－Ｇ＞ ＞ ＨＲＰ＞ ＣＳ．反应时间对试验结果有显著（Ｐ＜ ０．０５）影响。适当增加反应物浓度和延长反应时间对放大系统是有利的
The effects of glucose oxidase (GOD), β-D-G, horseradish peroxidase (HRP), chromogen (CS) and pH on the amplification system were studied. The results showed that the optimal pH value of the amplification system was 6.4, and the ideal chromogen was diphenylamine, 3,3 ’, 5,5’-tetramethylbenzidine (TMB) and o-phenylenediamine ) Should not be used as a color system to enlarge the original. The results of GOD, β-D-G, HRP and CS were all significantly (P <0.01) different in varying degrees. The primary and secondary relationships were GOD> β-D-G> HRP> CS. The reaction time had a significant (P <0.05) effect on the test results. Appropriately increasing the reactant concentration and extending the reaction time is advantageous for the amplification system