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目的通过DEK基因沉默对CaSki细胞生长、衰老的影响,探讨DEK原癌基因在宫颈癌发生中的作用。方法将DEKsiRNA真核表达载体转入CaSki细胞,观察转染后48 h细胞形态学变化,测定细胞生长曲线、SA-β-半乳糖苷酶细胞化学染色鉴定细胞衰老。结果与未转染和转染psiRNA-hH1neo组相比,转染psiRNA-hHDEK组CaSki细胞的生长速度明显降低;psiRNA-hHDEK转染组SA-β-半乳糖苷酶染色阳性细胞百分率(83.2±9.46)%高于转染psiRNA-hH1neo组(18.9±6.46)%和未转染CaSki组(23.1±7.28)%,结果具有统计学意义(P<0.05)。结论 DEK基因沉默后CaSki细胞发生衰老,证明DEK基因在宫颈癌中是衰老抑制基因。
Objective To study the effect of DEK gene silencing on the growth and senescence of CaSki cells and to investigate the role of DEK proto-oncogene in cervical carcinogenesis. Methods The eukaryotic expression vector of DEK siRNA was transfected into CaSki cells. The morphological changes of cells were observed 48 h after transfection. The cell growth curve was determined and the cell senescence was identified by SA-β-galactosidase cytochemical staining. Results Compared with the untransfected and transfected psiRNA-hH1neo groups, the growth rate of CaSki cells transfected with psiRNA-hHDEK group was significantly decreased. The percentage of SA-beta-galactosidase positive cells in psiRNA-hHDEK transfected group was 83.2 ± 9.46)% higher than that in the transfected psiRNA-hH1neo group (18.9 ± 6.46)% and the untransfected CaSki group (23.1 ± 7.28)%, the results were statistically significant (P <0.05). Conclusion The aging of CaSki cells after DEK gene silencing proves that DEK gene is an aging suppressor gene in cervical cancer.