论文部分内容阅读
乳酸脱氢酶(lacrate dehydrogenaseLDH)是细胞质的标志酶。此酶在心肌细胞中含量高。显示此酶的方法是根据细胞中的乳酸在LDH作用下氧化脱氢,经NAD(辅酶I)传递、使四唑盐还原生成兰紫色甲(月替)沉淀的原理制定的。几年来我们对培养大鼠心肌细胞的LDH作了大量观察,形成了稳定的LDH组化
Lactate dehydrogenase (lacrate dehydrogenaseLDH) is a cytoplasmic marker enzyme. This enzyme is high in cardiomyocytes. The method of displaying this enzyme is based on the principle that the lactate in the cell is oxidized and dehydrogenated under the action of LDH, and is transferred by NAD (Coenzyme I) to reduce the tetrazolium salt to form a purple purple (monthly) precipitate. Over the past few years we cultured rat cardiomyocyte LDH made a lot of observations, the formation of a stable LDH group