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目的:提取、鉴定陵水暗罗根提取物中挥发性成分,并评价其体外生物活性。方法:采用超临界CO2萃取法提取陵水暗罗根中的挥发性化学成分,气相色谱-质谱联用(GC-MS)法分析其化学成分组成;以0(空白对照)、5、10、20、30、40、50μg(生药)/ml提取物培养人肝癌Huh-7细胞24 h后,采用MTT法测定其对细胞的抑制作用,计算相对细胞活力和半数抑制浓度(IC50);分别采用纸片琼脂扩散法和肉汤稀释法测定其对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和粪肠球菌等菌株的体外抗菌活性。结果:从陵水暗罗根超临界CO2萃取物中分离鉴定了40个化合物,主要成分为(Z,Z,Z)-9,12,15-octa-decatrien-1-ol、甘油、肉桂醛、棕榈酸和丁香酚等;与空白对照组比较,陵水暗罗根提取物在5μg(生药)/ml以上质量浓度时对细胞生长有显著抑制作用(P<0.05),且随着质量浓度的增加抑制作用增强,IC_(50)值为5.2μg(生药)/ml;两种抑菌试验均未检测出其具有抑菌活性。结论:超临界CO_2萃取法和GC-MS法能有效提取和鉴定陵水暗罗根超临界CO_2萃取物的挥发性成分;提取物表现出抑制细胞活力的作用,但无抗菌活性。
OBJECTIVE: To extract and identify the volatile components in the extract of Haliotis concordiae and to evaluate its in vitro bioactivity. Methods: The chemical constituents were extracted by supercritical CO2 extraction and analyzed by gas chromatography-mass spectrometry (GC-MS). The chemical constituents were identified by 0 (blank control), 5, 10, Huh-7 cells were cultured with 20, 30, 40, 50μg (crude drug) / ml extract for 24 h, MTT assay was used to determine the inhibitory effect on the cells and the relative cell viability and IC50 were calculated. In vitro antibacterial activity against Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus and Enterococcus faecalis were determined by disk diffusion and broth dilution methods. Results: Forty compounds were isolated and identified from the supercritical carbon dioxide extract of Haliotis concinnus. The main constituents were (Z, Z, Z) -9,12,15-octadecadien-1-ol, Acid and eugenol. Compared with the blank control group, the extract of Haliotis divertin had a significant inhibitory effect on the cell growth (P <0.05) when its concentration was above 5μg / ml (P <0.05), and with the increase of mass concentration The inhibitory effect was enhanced with an IC 50 value of 5.2 μg / ml. Both antibacterial tests showed no antibacterial activity. Conclusion: The supercritical CO_2 extraction method and GC-MS method can effectively extract and identify the volatile components of the supercritical CO_2 extract of H. indica. The extract shows the effect of inhibiting cell viability but has no antibacterial activity.