论文部分内容阅读
采用高效液相色谱法,以Shimpak DIOL—150(250×7.9mm id)色谱柱分离纯化经过预处理的人体血、尿标本中的白蛋白。血清标本先用饱和硫酸铵溶液沉淀蛋白,再经透析浓缩后上柱分离;尿标本经过滤后直接透析浓缩即可上柱。本法具有效率高、纯度高的特点。
HPLC was used to separate and purify albumin in pre-treated human blood and urine samples on a Shimpak DIOL-150 (250 x 7.9 mm id) column. Serum samples were first precipitated with saturated ammonium sulfate solution, then separated by dialysis and concentrated on the column; urine samples were filtered and directly concentrated on the column. This method has the characteristics of high efficiency and high purity.