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目的:探讨益气养阴清热利湿方药对IgA肾病大鼠的治疗作用及对肾脏局部激肽释放酶-激肽系统系统的影响。方法:将SD大鼠随机分为4组,对照组、模型组、西药组、中药组,各组按相应药物剂量灌胃;造模前和第7、9、13、17周末检测24h尿蛋白定量,第17周末行肾组织IgA免疫荧光检测,并应用RT-PCR法检测肾组织KLK1和BK的表达水平。结果:与模型组比较,中药组、西药组IgA免疫荧光强度显著下降,KLK1和BK的表达水平显著增高(P<0.05)。结论:益气养阴清热利湿方药可能是通过激活肾脏局部KKS,达到延缓肾脏病理进展,发挥对IgA肾病的治疗作用。
Objective: To investigate the therapeutic effect of Yiqi Yangyin Qingre Lishi prescription on IgA nephropathy rats and its effect on the local kallikrein-kininogen system. Methods: The SD rats were randomly divided into 4 groups: control group, model group, western medicine group and traditional Chinese medicine group. Each group was given intragastric administration of the corresponding drug dose; 24h urinary protein Quantitative, 17th weekend kidney IgA immunofluorescence detection and RT-PCR method to detect the expression of KLK1 and BK in renal tissue. Results: Compared with model group, the immunofluorescence intensity of IgA in Chinese medicine group and western medicine group decreased significantly, while the expression of KLK1 and BK increased significantly (P <0.05). Conclusion: Yiqi Yangyin clearing heat and dampness prescriptions may delay renal pathological progress and activate IgA nephropathy through activation of local KKS.