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目的为探明中国人胃癌的微卫星ＤＮＡ的不稳定性（ｍｉｃｒｏｓａｔｅｌｉｔｅｉｎｓｔａｂｉｌｉｔｙ，ＭＳＩ）频率。方法选择了２９个多态微卫星标记，采用ＰＣＲ－聚丙烯酰胺凝胶电泳及银染技术对４２例胃癌组织进行了ＭＳＩ分析。结果中国人胃癌，在２９个位点上平均ＭＳＩ频率为３３．９％。在Ｄ３Ｓ１０６７、Ｄ３Ｓ１５７７、Ｄ８Ｓ２７９、Ｄ９Ｓ２５７、Ｄ１Ｓ２４８、Ｄ７Ｓ５２０及Ｄ２Ｓ１４７等位点上存在高频率的ＭＳＩ，其中Ｄ３Ｓ１５７７和Ｄ３Ｓ１０６７（５１．３５％）ＭＳＩ发生频率最高。胃癌的不同病理类型表现出不同的ＭＳＩ。低分化胃癌和印戒细胞癌与高分化癌比，ＭＳＩ频率明显增高（Ｐ＜０．０１）；而低分化胃癌和印戒细胞癌之间ＭＳＩ无显著差异（Ｐ＞０．０５）。结论ＭＳＩ在中国人胃癌中起着重要作用，尤其在低分化胃癌中。这些资料进一步证明，高低分化的胃癌可能具有不同的发病机理
Objective To explore the frequency of microsatellite DNA instability (MSI) in Chinese gastric cancer. Methods Twenty-nine polymorphic microsatellite markers were selected. MSI analysis was performed on 42 gastric cancer tissues by PCR-polyacrylamide gel electrophoresis and silver staining. Results In Chinese patients with gastric cancer, the mean frequency of MSI was 33.9% at 29 sites. High-frequency MSI existed in D3S1067, D3S1577, D8S279, D9S257, D1S248, D7S520 and D2S147, among which D3S1577 and D3S1067 (51.35%) had the highest frequency of MSI. Different pathological types of gastric cancer show different MSI. The frequency of MSI was significantly higher in poorly differentiated gastric cancer and signet-ring cell carcinoma than in well-differentiated cancer (P<0.01), while there was no significant difference in MSI between poorly differentiated gastric cancer and signet-ring cell carcinoma (P>0.05). Conclusion MSI plays an important role in Chinese gastric cancer, especially in poorly differentiated gastric cancer. These data further prove that high and low differentiation gastric cancer may have different pathogenesis