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白癜风丸是治疗白癜风的常用药物，本文通过双波长薄层扫描法测定了白癜风大蜜丸中主要有效成分补骨脂素和异补骨脂素的含量。选定波长、补骨脂素λ＿ｓ＝３１０ｎｍ，异补骨脂素λ＿ｓ＝３１０ｎｍ，λ＿Ｒ＝２１０ｎｍ。以苯：乙酸乙酯（１０．５：０．５）为展开剂。外标二点法计算含量，测得的补骨脂素和异补骨脂素的回收率分别为９９．２３％和９９．８０％。相对标准偏差分别为０．３４％和０．１２％。表明该法操作简便，分析迅速，结果准确。
Baijufeng Pill is a commonly used drug for the treatment of vitiligo. The content of psoralen and isopsoralen, which are the main active components in the Vitiligo Damai Pill, was determined by dual-wavelength thin-layer scanning. The selected wavelength, psoralen λ_s=310 nm, isopsoralen λ_s=310 nm, and λ_R=210 nm. Detergent was benzene:ethyl acetate (10.5:0.5). The external standard two-point method was used to calculate the content. The recoveries of the measured psoralen and isopsoralen were 99.23% and 99.80%, respectively. The relative standard deviations were 0.34% and 0.12%, respectively. It shows that this method is simple, rapid, and accurate.